Rishwanth Raghu will present his MSE talk "Tools for analysis of large scale cryo-EM and cryo-ET data" Thursday, April 25 at 1:00 PM in CS 301. Advisor: Ellen Zhong, Reader: Ben Raphael Abstract: Cryo-electron microscopy (cryo-EM) is a powerful technique for visualizing the structure and dynamics of macromolecules in near-physiological conditions. Owing to cryo-EM's ability to image biomolecules in varying native conformations, methods are increasingly being developed to reconstruct heterogeneous ensembles of 3D biomolecular structures from 2D cryo-EM image datasets. However, the field lacks standardized benchmarks for quantitative evaluation of these methods. We present CryoBench, a set of challenging synthetic cryo-EM datasets and novel metrics for the evaluation of heterogeneous reconstruction methods, along with benchmarking analysis of existing state-of-the-art methods. Next, we explore the semantic segmentation task for cryo-electron tomography (cryo-ET), a related biophysical technique for visualizing macromolecules within their cellular environments. We present work towards the generalizable segmentation of large-scale cryo-ET data given only sparse manual annotations. CS Grad Calendar: https://calendar.google.com/calendar/event?action=TEMPLATE <https://calendar.google.com/calendar/event?action=TEMPLATE&tmeid=MW1hanY5MD Q1bGRhaHF1NmVpcHRqMnBzZ2kgYWNnMDc5YmxzbzRtczNza2tmZThwa2lyb2dAZw&tmsrc=acg07 9blso4ms3skkfe8pkirog%40group.calendar.google.com> &tmeid=MW1hanY5MDQ1bGRhaHF1NmVpcHRqMnBzZ2kgYWNnMDc5YmxzbzRtczNza2tmZThwa2lyb 2dAZw&tmsrc=acg079blso4ms3skkfe8pkirog%40group.calendar.google.com